Technical Data
E3098-29T
Endothelin-1, Big (Big Endothelin 1)
Description:
Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Immunohistochemistry: 1:1000
Immunohistochemistry (PAP or ABC): 1:500
Optimal dilutions to be determined by the researcher.

AA Sequence:
Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Val-Asn-Thr-Pro-Glu-His-Val-Val-Pro-Tyr-Gly-Leu-Gly-Ser-Pro-Arg-Ser

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Endothelin-1, Big.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity:
Recognizes human Endothelin-1, Big. Species crossreactivity: Human Big Endothelin 1 (aa22-38). Species sequence homology ( 2%): Porcine Big Endothelin 2 (aa2-39), porcine Big Endothelin. Sequence homology (0%): Endothelin 1 (human, rat, porcine, mouse, canine, bovine), Endothelin 2 (human,canine), Endothelin 3 (human, rat), human BNP-32, Sarafotoxin S6b.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.