		Technical Data
E3100-01E	Endothelin A Receptor (Endothelin Receptor Type A, EDNRA, ETA, ET-A, ETAR, ETA-R, ETRA, Endothelin-1 Receptor, hET-AR)

Description:
The ETA receptor is an Endothelin Receptor that mediates the effects of endothelin-1, a mitogen with potent contractile properties. In the Human fetal olfactory epithelium cell line FNC-B4, the ETA receptor increases the secretion of gonadotropin-releasing hormone. Increased ETA concentration may be correlated with smooth muscle hypertrophy observed in patients suffering from bladder-outlet obstruction. ETA receptor has been reported in a variety of tissues including placenta, uterus, testis, heart, adrenal gland, lung, kidney, ovary, skin, liver, bladder, large blood vessels, cerebellum, neurons, and meningioma tumor cells. A splice variant has been identified in melanoma (Zhang et al., 1998). ESTs have been isolated from libraries from the above-mentioned tissues.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Heart, Cardiac myocytes

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to the 2nd extracellular domain of human Endothelin A Receptor.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.7, 0.01% sodium azide.

Specificity:

Recognizes human Endothelin A Receptor. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.