Technical Data
E3222-75N
Enolase 1 (ENO1, 2-phospho-D-glycerate Hydro-lyase, alpha Enolase, C-myc Promoter-binding Protein, ENO1L1, MBP-1, MBPB1, MPB1, Non-neural Enolase, NNE, Phosphopyruvate Hydratase, PPH, Plasminogen-binding Protein)
Description:
Enolase 1 gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen, paraffin): 1:25-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanRabbit
Concentration:
~5mg/ml
Immunogen:
Native human Enolase 1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human Enolase 1. Demonstrates < 0.1% crossreactivity with non-specific enolase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.