Technical Data
E3310-01B
Enterovirus
Description:
Enterovirus primarily infects the alimentary tract and probably infects other organs through the blood vascular systems. This antibody recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:10
Western Blot: 1:100-1:200
Immunocytochemistry: 1:20-1:40 for 30 min. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Immunofluorescence: Acetone-fixed HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern)
Western Blot: Sup T cells
Immunocytochemistry: Formalin-fixed, paraffin-embedded HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a5-D8/1Supernatant
SizeStorageShippingSourceHost
1ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Coxsackie virus B5.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Specificity:
Recognizes Enterovirus VP1 peptide. Recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Trabelsi A, Grattard F, Nejmeddine, et al.. Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody, 5-D8/1, in comparison with neu- tralization and PCR for rapid identification of enteroviruses in cell culture. Journal of Microbiology. 33:2454-2457 (1995). 2.Yousef G E, Bell E J, Mann G F, et al.. Chronic enterovirus infection in patients with postviral fatigue syndrome. The Lancet. i:146-150 (1988). 3.Yousef G E, Brown I N, and Mowbray J F. Derivation and biochemical characterization of an enterovirus group-specific monoclonal antibody. Intervirology. 28:163-170 (1987). 4.Yousef G E, Mann G F, Brown I N, et al.. Clinical and research application of an enterovirus group-reactive monoclonal antibody. Intervirology. 28:199- 205 (1987).