Technical Data
E3355-01B
EphA1 (Ephrin A1 receptor, Ephrin type-A receptor 1)
Description:
EPH receptor A1 (EphA1), with 976aa protein (~107kD), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date and their ligands, the ephrins,can be subdivided into two major subclasses, ephrin-A and ephrin-B. Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior, particularly in the nervous system. They have been implicated in various developmental processes, including axonal guidance, angiogenesis, morphogenesis and carcinogenesis.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen and paraffin): 1:200-1:1000
ELISA: 1:10,000
Western Blot: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18k50Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Ni-NTA purified truncated recombinant EphA1 expressed in E. coli strain BL21 (DE3).
Purity:
Purified
Form
Supplied as a liquid, 0.03% sodium azide.
Specificity:
Recognizes human Ephrin A1 receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Duffy, S.L., et al., (2006) Tam Gene Expr Patterns. Feb 6. 2. Pasquale, E.B. (2005) Nat Rev Mol Cell Biol. Jun; 6(6):462-75.