Technical Data
E3360-01J
Eck (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph receptor tyrosine kinase A2)
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Technical Data |
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E3360-01J |
Eck (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph receptor tyrosine kinase A2) |
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Description: Eck belongs to the Eph tyrosine receptor familiy, the largest receptor tyrosine kinase family of transmembrane proteins. It encodes a 130kD transmembrane protein which is primarily found in adult human epithelial cells (1). Eph receptors and their ephrin ligands are important mediators of cell-cell communication and play roles in embryonic patterning, neuronal targeting, and vascular development during normal embryogenesis (2,3). The Eph family of receptor tyrosine kinases is frequently overexpressed in a wide variety of cancers and tumor cell lines. In particular, Eck is overexpressed in prostate, lung and colon cancers and 40% of breast cancers and it represents an attractive potential target for drug design (3,4). Applications: Suitable for use in ELISA, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 1:200-1:400 Flow Cytometry: 1.2ug/10e6 cells Immunnofluorescence: 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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