Technical Data
E3360-01J
Eck (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph receptor tyrosine kinase A2)
Description:
Eck belongs to the Eph tyrosine receptor familiy, the largest receptor tyrosine kinase family of transmembrane proteins. It encodes a 130kD transmembrane protein which is primarily found in adult human epithelial cells (1). Eph receptors and their ephrin ligands are important mediators of cell-cell communication and play roles in embryonic patterning, neuronal targeting, and vascular development during normal embryogenesis (2,3). The Eph family of receptor tyrosine kinases is frequently overexpressed in a wide variety of cancers and tumor cell lines. In particular, Eck is overexpressed in prostate, lung and colon cancers and 40% of breast cancers and it represents an attractive potential target for drug design (3,4).

Applications:
Suitable for use in ELISA, Flow Cytometry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
ELISA: 1:200-1:400
Flow Cytometry: 1.2ug/10e6 cells
Immunnofluorescence: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19E89Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
cDNA encoding human Eck.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes native human Eck expressed on transfected cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.