		Technical Data
E3360-01L	Eck/EphA2 (EPHA2, EPH receptor A2, ARCC2, ECK, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK) (PE)

Description:
EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.

Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:5. Dilute with PBS. 10ul labels 1x10e6 in 100ul.
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 cells

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG1	11C238	Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C Do Not Freeze	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Native protein isolated by purification of phosphotyrosine- containing proteins.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 15mg/ml BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

Specificity:

Recognizes human Epithelial Cell Kinase (Eck/EphA2).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Carles-Kinch, K., et al. (2002). Cancer Res. 62:2840-7. 2. Hess, A.R., et al. (2001). Cancer Res. 61:3250-5. 3. Kinch, M.S., et al. (1998). Hybridoma. 17:227- 35. 4. Magal, E., et al. (1996). J. Neurosci. Res. 43:735-744. 5. Romer, L., et al. (1994). Mol. Biol Cell. 5:349- 361.