Technical Data
E3371-03B
EphB2 Receptor (Ephrin B2 Receptor)
Description:
The Eph receptors are the largest family of receptor tyrosine kinases. They can be grouped into two subclasses based on sequence homology: the EphA and the EphB receptors. The ligands for the Eph receptors are the ephrins. The EphA receptors bind ephrin-A ligands, which are tethered to the membrane by a GPI linkage and the EphB receptors bind ephrin-B ligands, which have a membrane spanning region. The Eph receptors are widely expressed during embryonic
development, including in the nervous system and the vascular system. Ephrins and Eph receptors have been implicated in many developmental processes including neuronal network formation, guidance of cell migration and axonal pathfinding. They remain expressed at lower levels in adult tissues and are upregulated in pathological conditions such as cancer. EphB2 is expressed on migrating neural crest cells and spinal motor neurons, as well as on cells of the anterior somite. EphB2 is also expressed in the visual system and in hippocampal neurons. In tumor tissues compared with matched normal tissues, EphB2 expression and overexpression has been reported in colon carcinomas and gastrointestinal tumor specimens, respectively. EphB2 has previously been designated Erk, Hek5 and Drt in human; Nuk and Sek3 in mouse;
Cek5 and Qek5 in avian species.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml
Immunoprecipitation: 5ug
Optimal dilutions to be determined by the researcher.

Positive Control:
EphB2-transfected 293T cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k6A75Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal region of the recombinant EphB2 protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the EphB2 receptor protein at ~110-120kD. Species Crossreactivity: chicken. Species Sequence Homology: mouse and human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Holder N, et al. Development 126(10):2033-2044, 1999. 2. Flanagan JD, et al. Annu Rev Neurosci 21:309-345, 1998. 3. Pasquale EB. Curr Opin Cell Biol 9(5):608-615, 1997. 4. Eph Nomenclature Committee. Cell 90(3):403-4, 1997. 5. Liu W, et al. Cancer 94(4):934-939, 2002. 6. Kataoka H, et al. J Cancer Res Clin Oncol 128(7):343-348, 2002.