Technical Data
E3371-04
EphB2 (EPH receptor B2, CAPB, DRT, Ephrin type-B receptor 2, EPHT3, EPTH3, ERK, Hek5, HEK5, MGC87492, PCBC, Receptor protein-tyrosine kinase HEK5, Renal carcinoma antigen NY-REN-47, Tyro5, TYRO5, Tyrosine-protein kinase receptor EPH-3, Tyrosine-protein ki
Description:
EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a 125kD member of the transmembrane Eph receptor tyrosine kinase family. The 90kD extracellular domain (ECD) can be shed from neurons by calcium ionophores or NMDA receptor agonists. EphB2 interaction with Ephrin-A5 promotes axonal growth cone collapse, while its interaction with Ephrin-B ligands is required for inner ear, renal, urorectal, and vascular development. Within the ECD, mouse EphB2 shares 99% aa sequence identity with human and rat EphB2.

Applications:
Suitable for use in Direct ELISA, Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 25ug/ml, 10ul labels 1-5x10e5 cells in a total reaction volume 200ul. Tested using MDA-MB-231 cells.
Immunocytochemistry: 10ug/ml detects EphB2 in MDA-MB-231 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted E3371-04 followed by NL557-coupled anti-rat IgG in the dark. Between each step, cells were washed with PBS, BSA.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9H113Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
NS0-derived, recombinant mouse EphB2 extracellular domain (aa27-551; Accession #P54763).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes mouse EphB2. Species sequence homology: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.