Technical Data
E3371-04A
EphB2 (EPH receptor B2, CAPB, DRT, Ephrin type-B receptor 2, EPHT3, EPTH3, ERK, Hek5, HEK5, MGC87492, PCBC, Receptor protein-tyrosine kinase HEK5, Renal carcinoma antigen NY-REN-47, Tyro5, TYRO5, Tyrosine-protein kinase receptor EPH-3, Tyrosine-protein ki
Description:
EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a 125kD member of the transmembrane Eph receptor tyrosine kinase family. The 90kD extracellular domain (ECD) can be shed from neurons by calcium ionophores or NMDA receptor agonists. EphB2 interaction with Ephrin-A5 promotes axonal growth cone collapse, while its interaction with Ephrin-B ligands is required for inner ear, renal, urorectal, and vascular development. Within the ECD, mouse EphB2 shares 99% aa sequence identity with human and rat EphB2.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19H114Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~500ug/ml
Immunogen:
NS0-derived, recombinant mouse EphB2 extracellular domain aa27-551; P54763.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity:
Recognizes mouse EphB2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.