Technical Data
E3373-05C
EphB4 (Ephrin type-B receptor 4, HTK, MYK1, Tyro11, TYRO11)
Description:
Eph receptors belong to a family of receptor tyrosine kinases. Both ephrins, the ligands for Eph receptors, and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos. The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells, formation of boundaries between structures (i.e. rhombomeres and somites), and the control of cytoskeletal changes which dictate cellular shape and adhesion during development. The EphB4 receptor, also known as Htk, Myk1, Tyro11 and Mdk2, was originally identified in CD34+ cells in the bone marrow and has since also been found in erythroid progenitor cells. The interaction between the EphB4 receptor and its ligand, ephrin B2, is critical for embryonic capillary morphogenesis. Upregulation of the EphB4 receptor has been observed in colon carcinoma, and in endometrial cancer4.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6A77Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
0.5mg/ml
Immunogen:
Recombinant protein corresponding to the C-terminal sequence of the human EphB4 receptor.
Purity:
Purified by Protein A chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human EphB4 receptor protein. Reactivity is confirmed with human breast carcinoma cell line MDA-MB-231, and 293 cells carrying an expression vector encoding human EphB4 receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang Z, et al. Blood 99(8):2740-2747, 2002. 2. Liu W, et al. Cancer 94(4):934-9, 2002. 3. Stephenson SA, et al. BMC Mol Biol. 2(1):15, 2001. 4. Takai N, et al. Oncol Rep 8(3):567-573, 2001.