Technical Data
E3373-05D
EphB4 Receptor Tyrosine Kinase
Description:
The Eph receptors are the largest family of receptor tyrosine kinases. They can be grouped into two subclasses: the EphA and the EphB receptors. The ligands for the Eph receptors are the ephrins. The EphA receptors bind ephrin-A ligands, which are tethered to the membrane by a GPI linkage, and the EphB receptors bind ephrin-B ligands, which have a membrane spanning region. The Eph receptors are widely expressed during embryonic development. Ephrins and Eph receptors have been implicated in many developmental processes including neuronal network formation, guidance of cell migration, and axonal pathfinding. They remain expressed at lower levels in adult tissues and are upregulated in pathological conditions such as cancer. EphB4 is expressed in epithelial cells and in endothelial cells of embryonic veins, where its signaling mediates cell migration and proliferation via the PI3K pathway. It is upregulated in several types of tumors, including mammary tumors, colon cancer, and endometrial cancer. EphB4 has previously been designated Htk, Myk1, Tyro11 and Mdk2.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml
Immunoprecipitation: 5ug/IP reaction
Immunohistochemistry: 10ug/ml. Formalin-fixed, paraffin-embedded tissues require heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0 prior to staining.
Optimal dilutions to be determined by the researcher.

Positive Controls:
Western Blot: EphB4-transfected 293T cells and human MDA-MB-231 breast carcinoma cell lysates
Immunohistochemistry: Human colon cancer tissues

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k6A76Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein derived from the C-terminal region of the human EphB4 protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes endogenous human EphB4 receptor at ~110-120kD, with weak crossreactivity with the EphB2 receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Holder, N., et al., Development 126(10): 2033-2044 (1999). 2. Flanagan, J.D., et al., Annu Rev Neurosci 21: 309-345 (1998). 3. Steinle, J.J., et al., J. Biol. Chem. 277(46): 43,830-43,835 (2002). 4. Eph Nomenclature Committee Cell 90(3): 403-404 (1997). 5. Pasquale, E.B., Curr. Opin. Cell Biol. 9(5): 608-615 (1997). 6. Berclaz, G., et al., Oncol. Rep. 9(5): 985-989 (2002). 7. Stephenson, S.A., et al., BMC Mol Biol 2(1): 15 (2001). 8. Berclaz, G., et al., Ann. Oncol. 14(2): 220-226 (2003).