Technical Data
E3373-05F
EphB4 Receptor Tyrosine Kinase (Eph Receptor B4, EphB4, Hepatoma Transmembrane Kinase, HTK, MYK1, TYRO11)
Description:
EphB4 is a member of the Eph receptor family, which bind the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19G66Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
As reported
Immunogen:
Recombinant mouse EphB4 extracellular domain
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in PBS.
Specificity:
Recognizes mouse EphB4. In this format, this antibody does not crossreact with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 or EphB6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.