Technical Data
E3373-05K
EPHB4 (Ephrin type-B Receptor 4, Tyrosine-protein Kinase Receptor HTK, Tyrosine-protein Kinase TYRO11, HTK, TYRO11)
Description:
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Western Blot: 1:200-1:2.000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10i39Affinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
EPHB4 recombinant protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human EPHB4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Steinle, J.J., et al., J. Biol. Chem. 277(46):43830-43835 (2002). 2.Suenobu, S., et al., Biochem. Biophys. Res. Commun. 293(3):1124-1131 (2002). 3.Wang, Z., et al., Blood 99(8):2740-2747 (2002). 4.Wilson, M.D., et al., Nucleic Acids Res. 29(6):1352-1365 (2001). 5.Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).