Technical Data
EPHB4 (Ephrin type-B Receptor 4, Tyrosine-protein Kinase Receptor HTK, Tyrosine-protein Kinase TYRO11, HTK, TYRO11) (Azide free) (HRP)
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Western Blot: 1:200-1:2.000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
MabIgG1,k10i39Affinity Purified
200ul-20CBlue IceHumanMouse
As reported
EPHB4 recombinant protein.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Recognizes human EPHB4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Steinle, J.J., et al., J. Biol. Chem. 277(46):43830-43835 (2002). 2.Suenobu, S., et al., Biochem. Biophys. Res. Commun. 293(3):1124-1131 (2002). 3.Wang, Z., et al., Blood 99(8):2740-2747 (2002). 4.Wilson, M.D., et al., Nucleic Acids Res. 29(6):1352-1365 (2001). 5.Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).