Technical Data
E3373-05K-HRP
EPHB4 (Ephrin type-B Receptor 4, Tyrosine-protein Kinase Receptor HTK, Tyrosine-protein Kinase TYRO11, HTK, TYRO11) (Azide free) (HRP)
Description:
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Western Blot: 1:200-1:2.000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
MabIgG1,k10i39Affinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanMouse
Concentration:
As reported
Immunogen:
EPHB4 recombinant protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.
Specificity:
Recognizes human EPHB4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Steinle, J.J., et al., J. Biol. Chem. 277(46):43830-43835 (2002). 2.Suenobu, S., et al., Biochem. Biophys. Res. Commun. 293(3):1124-1131 (2002). 3.Wang, Z., et al., Blood 99(8):2740-2747 (2002). 4.Wilson, M.D., et al., Nucleic Acids Res. 29(6):1352-1365 (2001). 5.Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).