Technical Data
EPHB4 (Ephrin type-B Receptor 4, Tyrosine-protein Kinase Receptor HTK, Tyrosine-protein Kinase TYRO11, HTK, TYRO11)
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceHumanRabbit
As reported
Human recombinant EphB4 protein.
Purified by ammonium sulfate precipitation.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human EphB4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Steinle, J.J., et al., J. Biol. Chem. 277(46):43830-43835 (2002). 2.Suenobu, S., et al., Biochem. Biophys. Res. Commun. 293(3):1124-1131 (2002). 3.Wang, Z., et al., Blood 99(8):2740-2747 (2002). 4.Wilson, M.D., et al., Nucleic Acids Res. 29(6):1352-1365 (2001). 5.Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).