Technical Data
Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF, Urogastrone)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant human epidermal growth factor(EGF) is a 6.3kD globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli.

AA Sequence:

Biological activity:
The ED50 for this effect is < 0.89 ng/ml . Measured in a cell proliferation assay using mouse Balb3T3 cell.

Activity Assay:
1. Cell line: Balb-3T3 (Mus musculus (mouse))
2. Maintenance Condition: RPMI 1640 containing 10% FBS
3. Assay medium - serum free RPMI 1640
4. Cell density: 2x10e4 cells/well (96 well plate, final volume 100ul)
5. Serum free starvation : 24hr with RPMI1640
6. Incubation time : 24 hr (after sample treatment)
7. Concentration range: 1 pg/ml - 1 ug/ml
8. Detection method : BrdU assay

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human, Recombinant, E. coli
Purity: 95% by SDS-PAGE. Purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Concentration: As reported
Form: Supplied as a liquid in PBS, pH 7.4.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Riese, et al., (1998) Bioessays 20:41-48. 2. Cohen, S., (1983) Cancer 15:1787-1791. 3. Carpenter, G., et al., (1979) Annu. Rev. Biochem. 48:193-216.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.