Technical Data
E3374-03F
Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF)
100ug
500ug
Growth Factors, Cytokines Storage: -70CShipping: Dry Ice
EGF was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kD precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active.

Biological Activity:
Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is 0.1 ng/ml. , corresponding to a specific activity of > 1.0x 10e7 units/mg.

AA sequence:
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Endotoxin: 1EU/ug as determined by LAL method.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
6222 D
Source: Escherichia coli
Purity: ~98% by SDS-PAGE and HPLC analyses.
Form:Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitution: Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.