Technical Data
E3374-03J
Epidermal Growth Factor (21-Leu), Recombinant, Human
50ug
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Technical Data |
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E3374-03J |
Epidermal Growth Factor (21-Leu), Recombinant, Human |
50ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Growth factor additive in cell culture media for keratinocytes, epithelial and epidermal cells. Half maximum stimulation of cell proliferation with MDCK cells is reached using < 10ng/ml. Biological Activity: 10ng/ml for half maximum stimulation of cell proliferation with MDCK cells. Applications: Characterized growth factor additive in cell culture media for keratinocytes, epithelial and epidermal cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 6205 |
Source: Human recombinant, produced in E. coli Purity: 98%; HPLC (reverse phase). Concentration: ~0.5mg/ml Form: Supplied as a lyophilized powder from PBS. Reconstitute with 100ul dH2O.. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Barrandon Y and Green H, (1987) Cell 50:1131-1137. 2. Carpenter G and Zendegui J, (1985) Analyt. Biochem. 153:279-282. 3. Oka T, et al., (1985) Natl Acad Sci USA 82:7212-7216. 4. Sumi S-I, et al., (1985) Biotec 2:59-74. 5. McRoberts JA, (1981) Functionally Differentiated Cell Line. Gardon Sato, ed.117-139.
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