Technical Data
E3374-03K
Epidermal Growth Factor (21-Leu), Recombinant, Human
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Growth factor additive in cell culture media for keratinocytes, epithelial and epidermal cells. Half maximum stimulation of cell proliferation with MDCK cells is reached using < 10 ng/ml.

Biological Activity:
10 ng/ml for half maximum stimulation of cell proliferation with MDCK cells.

Applications:
Suitable for use as a characterized growth factor additive in cell culture media for keratinocytes, epithelial and epidermal cells.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
6205
Source: Human recombinant, produced in E. coli
Purity: ~ 98%; HPLC (reverse phase).
Concentration: ~0.5mg/ml
Form: Supplied as a lyophilized powder from PBS, pH 6. Reconstitute with 200ul ddH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Barrandon Y and Green H, (1987) Cell 50:1131-1137. 2. Carpenter G and Zendegui J, (1985) Analyt. Biochem. 153:279-282. 3. Oka T, et al., (1985) Natl Acad Sci USA 82:7212-7216. 4. Sumi S-I, et al., (1985) Biotec 2:59-74. 5. McRoberts JA: The madin darby canine kidney (MDCK) cell line. In : Functionally Differentiated Cell Line. Gardon Sato, ed. 117-139 (1981).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.