Technical Data
E3374-11
Epidermal Growth Factor, Recombinant, Mouse (EGF, beta-Urogastrone, URG)
100ug
500ug
Molecular Biology Storage: -20CShipping: Blue Ice
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant murine EGF is a 6.0kD protein containing 53 amino acid residues.

Amino Acid Sequence:
NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Biological Activity:
The ED50 as determined by a cell proliferation assay using BALB/c 3T3 cells is 0.1ng/ml, corresponding to a specific activity of 1x10e7 units/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
6kD
Source: E. coli.
Purity: 8% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug).
Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 8.2. Reconstitute sterile ddH2O to 0.5-1mg/ml, which can then be further diluted to other aqueous solutions

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.