Technical Data
Epidermal Growth Factor, Recombinant, Mouse (EGF, beta-Urogastrone, URG)
Molecular Biology Storage: -20CShipping: Blue Ice
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant murine EGF is a 6kD protein containing 53 amino acid residues.

Recombinant mouse Epidermal Growth Factor, aa977-1029, expressed in E. coli.

Molecular Weight: 6kD

Biological Activity:
ED50 is 0.1-0.2ng/ml as determined by a cell proliferation assay using BALB/c 3T3 cells.

Endotoxin: 1EU/ug (LAL)

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% (SDS-PAGE and HPLC)
Concentration: As reported
Form: Supplied as a lyophilized powder. Reconstitute with ddH2O or PBS.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Suppressor of cytokine signaling-2 limits intestinal growth and enterotrophic actions of IGF-I in vivo Am. J. Physiol. Gastrointest. Liver Physiol. 291 (3), G472-G481. 2. The kinases MSK1 and MSK2 are required for epidermal growth factor-induced, but not tumor necrosis factor-induced, histone H3 Ser10 phosphorylation J. Biol. Chem. 281 (18), 12521-12525 (2006)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.