Technical Data
Epidermal Growth Factor, Recombinant, Rat (EGF)
Molecular Biology Storage: -20CShipping: Blue Ice
EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF- and VGF (vaccinia virus growth factor). Recombinant rat EGF is a 6.2kD globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds.

Biological Activity:
The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is < 0.1ng/ml, corresponding to a specific activity of >1x10e7 units/mg.

Amino Acid Sequence:

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, carrier protein. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
Source: E. coli
Purity: 98% by SDS-PAGE
Form: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O, carrier protein to a final concentration of 0.1-1mg/ml. Do not vortex.
Endotoxin:<0.1ng/ml (1EU/ug)

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.