Technical Data
E3375
Epidermal Growth Factor Receptor (erbB, EGFR)
Description:
Epidermal growth factor (EGF) receptor is a 170kD tyrosine kinase. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation. Phosphorylation of Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the enzyme in an active state and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of Tyr845. Phospho-tyrosine 992 is a direct binding site for the PLC gamma SH2 domain, resulting in activation of PLC gamma-mediated downstream signaling. Phosphorylation of Tyr1045 creates a major docking site for c-Cbl. Binding of c-Cbl to the activated EGFR leads to receptor ubiquitination and degradation. Phospho-Tyr1068 of activated EGFR is a direct binding site for Grb2. Phospho-tyrosine 1148 and 1173 provide a docking site for SHC. Both sites are involved in the activation of MAP kinase signaling. Phosphorylation of EGFR on serine and threonine residues attenuates EGFR kinase activity. Serines 1046 and 1047 in the carboxy-terminal region of EGFR are sites phosphorylated by CaM kinase II. Mutations to either serine 1046 or 1047 upregulate tyrosine autokinase activity of EGFR.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry: Formalin-fixed cells
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
A-431 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanSheep
Concentration:
~1mg/ml
Immunogen:
Recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.15M PBS, pH 7.4. No preservatives added.
Specificity:
Recognizes human Epidermal Growth Factor Receptor. Species Crossreactivity: rat, mouse and chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Davis, R.J., et al., Advances in Lipid Research 25: 131 (1993).