Technical Data
E3375-07
Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain
Description:
EGFR is a type I recpetor tyrosine kinase with sequence homology to erb-B 1, 2, 3, 4, or HER 1, 2, 3, 4. It binds to epidermal growth factor, transforming growth factor-a, heparin binding EGF, amphiregulin, betacellulin, and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml for 2 hours at RT
Immunoprecipitation (Native only): 2ug/mg protein lysate using Protein G.
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires digestion with protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 cells. Placenta or squamous cell carcinoma.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.N.269Affinity Purified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
HC2 20 d2 cells. Cellular Localization: Cell membrane.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before the addtion of glycerol to 40%. Also available without BSA and azide. See E3375-07X.
Specificity:
Recognizes the 170kD (wild type) and 145kD (VIII variant) forms of human EGF receptor. The epitope maps to the extracellular domain of EGFR. Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wikstrand CJ, et. al., (1995) Cancer Research 55(14):3140-8.