Technical Data
E3375-07X
Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain (BSA & Azide Free)
Description:
EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to epidermal growth factor (EGF), transforming growth factor-a (TGF-a), heparin binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is over expressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-1ug/ml for 2 hrs at RT. Suitable for Western blot of extracellular domain of EGFR.
Immunoprecipitation (Native only): 2ug/mg protein lysate. Use Protein G.
Immunohistochemistry (Formalin/ Paraffin): 2-4ug/ml for 30 mniutes at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 min at 37C. (Note: E3375-06B/ E3375-06BX are more suitable for use in IHC).
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 cells, placenta or squamous cell carcinoma.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k0.N.269Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
HC2 20 d2 cells. Cellular Localization: Cell membrane. Epitope: Extracellular domain.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservatives and no stabilizing proteins added. Also available with BSA and azide. See Cat # E3375-07.
Specificity:
Recognizes the 170kD (wild type) and 145kD (VIII variant) forms of human EGF receptor. Species Crossreactivity: Does not crossreact with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wikstrand, C.J., et. al., Cancer Research 55(14): 3140-8 (1995).