Technical Data
E3375-11
Epidermal Growth Factor Receptor (erbB, EGFR)
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.

Recommended Dilution:
Immunocytochemistry: 5ug/ml. Demonstrates positive immunostaining for EGF receptor in A431cells fixed with 4% paraformaldehyde followed by 100% methanol.
Western Blot: 0.5-2ug/ml. Detects EGF receptor (~180kD) in RIPA lysates from human A431 cells under reducing conditions. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3375-11 (1ug/ml). Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug. Immunoprecipitates EGF receptor from 500ug of A431 RIPA lysate.
Optimal dilutions to be determined by the researcher.

Positive Antigen Control:
A0001-50: A431 Cell Lysate, Non-Stimulated

Hybridoma:
X63-Ag8.653x Balb/c-spleen cells.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a0.T.36Affinity Purified
SizeStorageShippingSourceHost
250ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
A431 membrane proteins.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kD. Species crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wu, D., et al., (1989) J. Biol. Chem. 264:17469–17475. 2. Wu, D., et al., (1990) Proc. Natl. Acad. Sci. USA 87:3151–3155. 3. Miloso, M., et al., (1995) J. Biol. Chem. 270:19557–19562. 4. Brambilla, R., et al., (1995) EMBO J. 14:3116–3126.