Technical Data
E3375-11A1
EGFR (Epidermal Growth Factor Receptor, Receptor Tyrosine-protein Kinase ErbB-1, Proto-oncogene c-ErbB-1, ERBB1)
Description:
EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Applications:
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000
Western Blot: 1:2000
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10i41Affinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified His-tagged EGFR protein (fragment).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human EGFR.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aifa, S., et al., Exp. Cell Res. 302(1): 108-114 (2005). 2. Adams, T.E., et al., Growth Factors 22(2): 89-95 (2004). 3. Ichinose, J., et al., Biochem. Biophys. Res. Commun. 324(3): 1143-1149 (2004). 4. Kuribayashi, A., et al., Endocrinology 145(11): 4976-4984 (2004). 5. Kapoor, G.S., et al., Mol. Cell. Biol. 24(2): 823-836 (2004). 6. Hartl, D., et al. J. Biol. Chem. 283: 33,472-33,482 (2008). (Applications: WB)