Technical Data
E3375-25
Epidermal Growth Factor Receptor (erbB, EGFR)
Description:
Epidermal growth factor (EGF) receptor is a 170kD tyrosine kinase. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation (1,2). Phosphorylation of Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the enzyme in an active state and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of Tyr845 (5). Phospho-tyrosine 992 is a direct binding site for the PLC gamma SH2 domain, resulting in activation of PLC gamma-mediated downstream signaling (6). Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGFR leads to receptor ubiquitination and degradation (8). Phospho-Tyr1068 of activated EGFR is a direct binding site for Grb2 (9). Phospho-tyrosine 1148 and 1173 provide a docking site for SHC (2). Both sites are involved in the activation of MAP kinase signaling. Phosphorylation of EGFR on serine and threonine residues attenuates EGFR kinase activity. Serines 1046 and 1047 in the carboxy-terminal region of EGFR are sites phosphorylated by CaM kinase II. Mutations to either serine 1046 or 1047 upregulate tyrosine autokinase activity of EGFR (10).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to residues surrounding Tyr1068 of human EGF receptor.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Recognizes endogenous levels of total human EGF receptor protein at ~175kD. Does not crossreact with other proteins of the ErbB family. Species Crossreactivity: mouse, rat and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hackel, P.O., et al. (1999) Curr. Opin. Cell Biol. 1:184-189. 2. Zwick, E., et al., (1999) Trends Pharmacol. Sci. 20:408-412. 3. Cooper, J.A., Howell, B., (1993) Cell 73:1051-1054. 4. Hubbard, S.R., et al., (1994) Nature 372:746-754. 5. Biscardi, J.S., et al., (1999) J. Biol. Chem. 274:8335-8343. 6. Emlet, D.R., et al., (1997) J. Biol. Chem. 272:4079-4086. 7. Levkowitz, G., et al., (1999) Mol. Cell 4:1029-1040. 8. Ettenberg, S.A., et al., (1999) Oncogene 18:1855-1866. 9. Rojas, M., et al., (1996) J. Biol. Chem. 271:27,456-27,461. 10. Feinmesser, R.L., et al., (1999) J. Biol. Chem. 271:27,456-27,461. 11. Endo, A., et al., (2002) J. Biol. Chem. 277:23,747-23,754. 12. Tschumperlin, D.J., et al. (2004) Nature 429:83-86. 13. Rimoldi, V., et al., (2003) Oncogene 22:6054-6060. 14. Zhang, Y., et al., (2004) Experimental Therapuetics, Preclinical Pharmacology 10:3667-3677.