		Technical Data
E3376-25	Epigen, Recombinant, Human	5ug 25ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, heart and in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7kD transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant human Epigen is a 7.9kD monomeric protein, containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor.

Source:
E. coli

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile buffer, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Biological Activity:
Determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expected ED50 for this effect is 150-300ng/ml.

Country of Origin:
USA

Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses.
Concentration: As reported
Form: Supplied as a lyophilized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.