Technical Data
Epigen, Recombinant, Human
Molecular Biology Storage: -20CShipping: Blue Ice
Epigen was identified as an EGF family member by amino acid sequence homology (1). The sequence was obtained from mouse keratinocyte mRNA. Epigen is expressed in normal human mammary tissue and in some prostate and breast carcinomas (2). Epigen binding to known EGF receptors is with lower affinity when compared to EGF. However, Epigen appears to be superior in inducing cell proliferation (2). Epigen induces responses in MDA-MB-468 breast carcinoma cells. Epigen also has effects on prostate cells and vascular endothelial cells (2). Epigen binds to ErbB1/Her1 (2). Binding causes receptor homodimerization and heterodimerization with other Erb/Her receptors (2).

Determined in a MCF-10A cell proliferation assay. The ED50 of each lot is between 2-30ng/ml.

0.01ng endotoxin/1ug hEpigen.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions o be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile buffer. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by SDS-PAGE
Concentration: Not determined
Form: Supplied as lyophilized powder from 20mM citrate, pH 3, 100mM NaCl and 20ug BSA per 1ug hEpigen.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Strachan, L. et al. (2001) J Biol Chem 276, 18265-71. 2. Kochupurakkal, B.S. et al. (2005) J Biol Chem 280, 8503-12.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.