Technical Data
E3376-25C
Epigen, Recombinant, Human, Ala24-Ala104 (BSA Free)
10ug
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Epigen was identified as an EGF family member by amino acid sequence homology (1). The sequence was obtained from mouse keratinocyte mRNA. Epigen is expressed in normal human mammary tissue and in some prostate and breast carcinomas (2). Epigen binding to known EGF receptors is with lower affinity when compared to EGF. However, Epigen appears to be superior in inducing cell proliferation (2). Epigen induces responses in MDA-MB-468 breast carcinoma cells. Epigen also has effects on prostate cells and vascular endothelial cells (2). Epigen binds to ErbB1/Her1 (2). Binding causes receptor homodimerization and heterodimerization with other Erb/Her receptors (2).

Source:
Recombinant human Epigen (hEpigen) Ala24-Ala104 was produced in E. coli. Recombinant hEpigen does not have a Met on the amino terminus and has a calculated MW of 9,087. DTT-reduced and non-reduced protein migrate as 8kD polypeptides with non-reduced protein having slightly greater mobility due to intramolecular cystines. The expected amino-terminal AVTVT of recombinant hEpigen was verified by amino acid sequencing.

Bioactivity:
Determined in a MCF-10A cell proliferation assay. The ED50 of each lot is between 2-30ng/ml. The proliferation of MCF-10A cells treated with increasing concentrations of hEpigen was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.

Endotoxin
0.01ng endotoxin/1ug hEpigen.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: Extracts from MCF-10A cells untreated or treated with hEpigen for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit mAb (upper) and p44/42 MAPK (Erk1/2) rabbit mAb (lower).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile buffer. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by SDS-PAGE of 6ug reduced (+) and non-reduced (-) recombinant hEpigen.
Concentration: Not determined
Form: Supplied as lyophilized powder in 20mM citrate, pH 3, 100mM NaCl. No stabilizing proteins added.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Strachan, L. et al. (2001) J Biol Chem 276, 18265-71. 2. Kochupurakkal, B.S. et al. (2005) J Biol Chem 280, 8503-12.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.