Technical Data
E3376-75
Epiregulin, Recombinant, Human (EREG)
5ug
25ug
Molecular Biology Storage: -20CShipping: Blue Ice
Epiregulin is an EGF related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes and in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0kD transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0kD mature secreted sequence. Recombinant human Epiregulin is a 6.0kD monomeric protein, containing 50 amino residues, which corresponds to the mature secreted Epiregulin sequence.

AA Sequence:
MVAQVSITKC SSDMNGLYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL

Biological Activity:
The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is 0.3ug/ml in the presence of 15ng/ml of human IGF-II.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O and carrier protein. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
USA
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses.
Concentration: As reported
Form: Supplied as a lyophilized powder with no additives.
Reconstitute in ddH20, carrier protein to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.