Technical Data
E3376-75B
Epiregulin, Recombinant, Human, Val63-Leu108 (BSA Free) (EREG)
10ug
50ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
Epiregulin is a member of the EGF family that is highly expressed in many cancer-specific cells, cells of the placenta and macrophages (1,2). Epiregulin induces keratinocyte and corneal epithelial cell proliferation, oocyte maturation and may function in blastocyst implantation during pregnancy (3-5). Active soluble epiregulin is produced by proteolytic processing of a transmembrane precursor. Epiregulin is the only EGF family member known to bind to both the ErbB1/HER1 and ErbB4/HER4 EGF receptors (6). Epiregulin binding induces receptor homodi- merization or heterodimerization with other Erb/Her receptors. Epiregulin activates the ERK1/2, and PI3K/Akt pathways (1,3).

Source:
Produced in E. coli.

Molecular Characterization:
Based on amino acid sequencing, greater than 50% of recombinant hEpiregulin has a Met on the amino-terminal Val63 (MVSIT) and has a calculated MW of 5,406. The remainder starts at Val63 (VSITK). DTT-reduced and non-reduced protein migrate as 5 kDa polypeptides.

Endotoxin:
0.01 ng endotoxin/1ug hEpiregulin.

Bioactivity:
The bioactivity of recombinant hEpiregulin was determined in a MCF 10A cell proliferation assay. The ED 50 of each lot is between 0.10-0.85ng/ml.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Source: E. coli
Purity: 98% as determined by SDS-PAGE of 6ug reduced (+) and non-reduced (-) recombinant hEpiregulin.
Concentration: As reported
Form: Supplied as lyophilized powder from 0.22um filtered solution of 20mM citrate, pH 3.0 containing 100mM NaCl. No preservative added. No stabilizing proteins added. Reconstitute with sterile 20mM citrate, pH 3.0 or 20mM citrate, pH 3.0 containing protein to minimize absorption of hEpiregulin to surfaces. Solubilize for 30 minutes at RT with occasional gentle vortexing. Should be 50ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Toyoda, H. et al. (1997) Biochem J 326 ( Pt 1), 69-75, 2. Révillion, F. et al. (2008) Ann Oncol 19, 73-80, 3. Morita, S. et al. (2007) Mol Vis 13, 2119-28, 4. Shirakata, Y. et al. (2000) J Biol Chem 275, 5748-53, 5. Song, H. et al. (2000) Mol Endocrinol 14, 1147-61, 6. Riese, D.J. et al. (1998) J Biol Chem 273, 11288-94.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.