Technical Data
E3385-20
Epiligrin (Laminin 5), Inhibiting
Description:
Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation and ELISA. Other applicatons not tested.

Recommended Dilution:
Immunohistochemistry: Acetone fixed sections
Western Blot: Non-reducing
Optimal dilution determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q587Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human keratinocytes
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS. pH 7.1. No preservatives added.
Specificity:
Recognizes human Epiligrin (Laminin 5).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wayner, E.A., et al., J. Cell. Biol. 121(5): 1141-1152 (1993). 2. Sriramarao, P., et al., J. Biol. Chemistry 268(29): 22,036-22,041 (1993). 3. Wewer, U.M., et al., Lab Invest. 71(5): 719-730 (1994). 4. Gonzales, M., et al., Molecular Biology of the Cell 10: 259-270 (1999). 5. Goldfinger, L.E., et al., Journal of Cell Biology 141(1): 255-265 (1998).