Technical Data
Media
Epithelial Basal Medium WAJC 404 (Powder)
E3400
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Ammonium Metavandate 0.000585
Ammonium Molybdate•4H2O0.0012359
Calcium Chloride•2H2O14.43
Cupric Sulfate•5H2O0.0002497
Ferric Nitrate•9H2O1.21
Magnesium Chloride 57.18
Manganese Sulfate•5H2O0.00241
Nickel Chloride•6H2O0.00011885
Potassium Chloride111.75
Sodium Chloride 7592
Sodium Metasilicate•9H2O 0.1421
Sodium Phosphate Dibasic 284.2
Sodium Selenite•5H2O0.00789
Stannous Chloride•2H2O 0.0001128
Zinc Sulfate•7H2O0.14375
Amino Acids:
L-Alanine 8.9
L-Arginine174.2
L-Asparagine13.21
L-Aspartic Acid3.993
L-Cystine57.67
L-Glutamic Acid14.71
L-Glutamine876.0
Glycine
L-Histidine12.416
L-Isoleucine1.97
L-Leucine65.6
L-Lysine
L-Phenylalanine4.96
L-Proline34.53
L-Serine
L-Threonine11.91
L-Tryptophan3.06
L-Tyrosine2.72
L-Valine
Vitamins:
Biotin
Choline Chloride 13.96
Vitamin B120.4066
Folic Acid0.7945
Folinic Acid, Ca0.7945
Myo-Inositol18.02
Nicotinamide0.0366
D Pantothenate, Ca0.258
Pyridoxine•HCl0.0616
Riboflavin0.376
Thiamine•HCl0.337
Other:
Adenine 24.32
Thymidine0.07266
D-Glucose 1080
HEPES Free Acid6672.4
Lipoic Acid0.2063
Phenol Red, Na1.242
Putrescine•2HCl0.161
Sodium Acetate303.4
Sodium Pyruvate 11.0
Total:17.6g/liter
Basal Medium for the growth of epithelial cells other than keratinocytes. When supplemented with growth factors or fetal calf serum (0.2-2.0% dialyzed), WAJC 404 is selective for endothelial calls and detrimental to mesenchymal cells.


Directions per Liter: Dissolve 17.6g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white to pale orange, homogenous, free-flowing powder

Solubility: Light amber to yellow, clear, complete

pH: 6.6±0.4


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. McKeehan, W. L., Adams, P. S., and Rosser, M. P., (1984) Cancer Res. 44:1998-2010.