Technical Data
Epithelial (Human Breast) Serum Free Media (BRFF™ BMZERO)
Cell Culture Grade
Storage -70°C    Shipping Dry Ice

BRFF-BMZERO™ is a complete serum-free medium designed to grow human breast cells. This SFM was originally used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines. This medium is a good first choice when switching from serum containing medium to serum free or for the cultivation of primary cultures. For optimum cell attachment, use FNC Coating Mix®.

Ready-to-use with all necessary growth factors. Antibiotic-free.

Storage and Stability:
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months after receipt.

Form: Supplied as a liquid.

pH: 7.2-7.5 (5% CO2 atmosphere is recommended for culture).

Conductivity: 10-16mS

Sterility Test: Pass Specification

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iype, E.L., et al., (1998) Cytotechnology 26: 207-218.