Technical Data
Media
Epithelial (Human Breast) Serum Free Media (BRFF BMZERO)
E3400-05
Cell Culture Grade
Storage -70C    Shipping Dry Ice

BRFF-BMZERO is a complete serum-free medium designed to grow human breast cells. This SFM was originally used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines. This medium is a good first choice when switching from serum containing medium to serum free or for the cultivation of primary cultures. For optimum cell attachment, use FNC Coating Mix.

Ready-to-use with all necessary growth factors. Antibiotic-free.

Storage and Stability:
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.


Form: Supplied as a liquid.

pH: 7.2-7.5 (5% CO2 atmosphere is recommended for culture).

Osmolality: 270-300mOsM Unit

Functional Test: Growth of cell line 267-B1 to confluence. One vial containing 1-5x10e6 celss is quick thawed, washed with 10ml of medium, resuspended in 10ml of medium and applied to a T-25 flask pre-coated with FNC Coating Mix. The culture is incubated at 37C in a 5% CO2 incubator.

Sterility Test: Pass Specification


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iype, E.L., et al., (1998) Cytotechnology 26: 207-218.