		Technical Data
E3405-50	Epithelial Cell Surface Antigen (ESA, Adenocarcinoma-associated Antigen, CD326, CD326 Antigen, Cell Surface Glycoprotein Trop1, Cell Surface Glycoprotein Trop-1, CO-17A, CO17-1A, Epithelial Cellular Adhesion Molecule, Ep CAM, EpCAM, Ep-CAM, Epithelial Gly

Description:
The epithelial cell adhesion molecule (Ep-CAM) is a 38kD cell surface antigen otherwise known as CD326, MOC31 and Ber-EP4. CD326 is a type 1 transmembrane glycoprotein. It is expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen and paraffin):1:200 for paraffin sections. Requires protein digestion pretreatment (e.g. trypsin, pronase) of paraffin sections.
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Western Blot: Detects a band at ~38kD in HT-29 cell lysates.
Optimal dilutions to be determined by the researcher.

Positive Control (Immunohistochemistry):
Large bowel

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	10B1416	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

H69 cell line (small cell lung carcinoma).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes the human epithelial cell adhesion molecule (Ep-CAM). at ~38kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Koga, Y. et al. (2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads. Gastroenterol. Res. Pract. 2008: 605273. 2. Fillmore, C.M. et al. (2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling. Proc Natl Acad Sci U S A. 107: 21737-42. 3. Murakata, A. et al. (2011) Gene expression signature of the gross morphology in hepatocellular carcinoma. Ann Surg. 253: 94-100. 4. Winter, M. J. et al. (2003) The epithelial cell adhesion molecule (Ep - CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am. J. Pathol. 163: 2139 - 2148.