Technical Data
Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Human Epidermal Growth Factor is a single, non-glycosylated, polypeptide chain containing 53aa and a molecular weight of 6.22kD. The sequence of the first five N-terminal aa was determined to be Asn-Ser-Asp-Ser-Glu. N-terminal methionine has been completely removed enzymatically. Contains less than 1% dimers and aggregates.

Recombinant Human Epidermal Growth Factor expressed in E. coli.

Molecular weight: 6.22kD

The ED50 calculated by the dose-dependent proliferation of murine Balb/c 3T3 cells (measured by 3H-thymidine uptake) is <0.1ng/ml corresponding to a specific activity of 1x10e7 Units/mg.

Endotoxin: 0.1 ng/ug

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Stable for at least 6 months at -20C. Reconstitute with sterile deionized water to a concentration 100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% (HPLC, FPLC, SDS-PAGE).
Form: Supplied in lyophilized powder. No preservative added. Reconstitute with sterile deionized water to a concentration 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.