Technical Data
E3410A
Epidermal Growth Factor, Recombinant, Rat (EGF)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. EGF (Epidermal Growth Factor) is a type I transmembrane protein expressed by a variety of cells, which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival.

Source:
Purified recombinant rat EGF expressed in E.coli.

Activity:
The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is 0.1 ng/ml, corresponding to a specific activity of 1x10e7 units/mg.

Applications:
Suitable for use in Functional Assays. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Endotoxin:
1EU/ug.

Storage and Stability:
Lyophilized powder may be stored at-20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
6.2kD
Source: E. coli.
Purity: 98% by SDS PAGE and HPLC analysis.
Concentration: As reported
Form: Supplied as a lyophilized powder in PBS, pH 7.2, 1% BSA. Reconstitute with 1ml sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.