Technical Data
E3412
Epidermal Growth Factor, Recombinant, Human (EGF, beta-Urogastrone, URG)
100ug
|
Technical Data |
|
E3412 |
Epidermal Growth Factor, Recombinant, Human (EGF, beta-Urogastrone, URG) |
100ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
|
Human EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human. Source: EGF1-52 Biological Activity: The mitogenic activity of human EGF is measured by the stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. In addition, EGF activity has been determined by a receptor-binding assay (RBA) using A-431 cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: EGF1-52 Purity: Purified. 97% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis Concentration: As reported Form: Supplied as a lyophilized powder in PBS Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
1. Cohen, S. (1962) J, Biol. Chem. 237:1555 2. Gregory, H. (1985) J.Cell Science Supp.. 3:11 3.- George-Nascimento, C. et al. (1988) Biochemistry 27:797-802
|
||