Technical Data
Epidermal Growth Factor, Recombinant, Human (EGF, beta-Urogastrone, URG)
Molecular Biology Storage: -20CShipping: Blue Ice
Human EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human.


Biological Activity:
The mitogenic activity of human EGF is measured by the stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. In addition, EGF activity has been determined by a receptor-binding assay (RBA) using A-431 cells.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: EGF1-52
Purity: Purified. 97% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis
Concentration: As reported
Form: Supplied as a lyophilized powder in PBS

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Cohen, S. (1962) J, Biol. Chem. 237:1555 2. Gregory, H. (1985) J.Cell Science Supp.. 3:11 3.- George-Nascimento, C. et al. (1988) Biochemistry 27:797-802

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.