Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP
Recommended Positive Control Tissue: Normal Epithelia
Immunohistochemistry: (frozen)- 1:10-1:40, (paraffin)- 1:10-1:40
Optimal working dilutions to be determined by researcher.
Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
| As reported|
|Delipidated extract of human cream.|
|Tissue culture supernatant.|
|Tissue Culture Supernatant. Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.|
|Recognizes epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400kD.This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Gusev, Y., et al., (2001) American Journal of Pathology 159(1):63-69.|