Technical Data
E3414-15D
Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)
Description:
Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP

Recommended Positive Control Tissue: Normal Epithelia

Applications:
Immunohistochemistry: (frozen)- 1:10-1:40, (paraffin)- 1:10-1:40
Optimal working dilutions to be determined by researcher.

Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
TypeIsotypeCloneGrade
MabIgG2a3H2099Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Delipidated extract of human cream.
Purity:
Tissue culture supernatant.
Form
Tissue Culture Supernatant. Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400kD.This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gusev, Y., et al., (2001) American Journal of Pathology 159(1):63-69.