Technical Data
E3414-15E
Epithelial Membrane Antigen (EMA)
Description:
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:10 1:40 (frozen or paraffin). Paraffin sections do not require protein digestion pretreatment or antigen retrieval using heat treatment prior to staining. Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9B58Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Delipidated extract of human cream.
Purity:
Supernatant.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Specificity:
Recognizes human epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400kD. This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.