Technical Data
E3414-16A
Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)
Description:
MUC-1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:25-50 for 30 min. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.

Positive Control: MCF-7 cells, Breast carcinoma

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
A synthetic peptide from cytoplasmic tail of human MUC-1 protein.Epitope: aa239-255. MW of Antigen: 265-400kD. Cellular Localization: Cytoplasmic and cell membrane.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human MUC-1. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.