Technical Data
E3414-17
Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)
Description:
Epithelial Membrane Antigen, also referred to as EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin and MUC-1 is a glycoprotein of variable molecular weight, expressed by many glandular epithelial cells. Epithelia of breast, (acini and ducts), exocrine pancreas, bladder, gastrointestinal tracts, thyroid, endometrium and respiratory tract are generally EMA positive.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:25-1:50 (streptavidin:biotin)
Formalin-fixed, paraffin-embedded tissue sections can be stained successfully but require heat treatment to restore antigenicity, before staining. This involves exposure of tissue sections to boiling 0.01M citrate buffer, pH 6.0 for 25-30 minutes or for 2-3 minutes at elevated pressure.

Optimal dilutions to be determined by the researcher.

Source:
Tissue culture supernatant

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM1.B.763Highly Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.05mg/ml
Immunogen:
Purified human Epithelial Membrane Antigen
Purity:
Purifed by gel filtration following ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide, before the addition of 40%. glycerol.
Specificity:
Recognizes Human surface glycoprotein of approximately 54kD. Positive against all curent carcinoma cell lines.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Southgate, J., Trejdosiewicz, L.K. & Hodges, G.M. in Press.