Technical Data
E3414-20
Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)
Description:
Epithelial Membrane Antigen, also referred to as EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin and MUC-1 is a glycoprotein of variable molecular weight, expressed by many glandular epithelial cells. Epithelia of breast, (acini and ducts), exocrine pancreas, bladder, gastrointestinal tracts, thyroid, endometrium and respiratory tract are generally EMA positive.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 0.1-0.2ug/ml for 30 minutes at RT. No special pretreatment is required.
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Positive Control:
Breast or colon carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.273Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human milk fat globule membranes. Cellular Localization: Cytoplasmic and cell membrane. Epitope: DTRP
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes human epithelial membrane antigen at ~265-400kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gown AM. 1988. Human Pathol. 19:119. 2. Russo J and Russo IH. In: DeLellis RA, ed. Advances in Immunohistochemistry. New York: Raven Press, 431-475, 1988.