		Technical Data
E3414-20C	Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)

Description:
Epithelial Membrane Antigen, also referred to as EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin and MUC-1 is a glycoprotein of variable molecular weight, expressed by many glandular epithelial cells. Epithelia of breast, (acini and ducts), exocrine pancreas, bladder, gastrointestinal tracts, thyroid, endometrium and respiratory tract are generally EMA positive.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Breast carcinoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	11E184	Supernatant

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined.

Immunogen:

Delipidated extract of human cream. Cellular Localization: Cell membrane and cytoplasmic.

Purity:

Supernatant

Form

Supplied as a liquid, 0.05% sodium azide.

Specificity:

Recognizes human EMA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Heyderman et al. Br J Cancer 52: 355, 1985. 2. Cordell et al. Br J Cancer 52: 347, 1985. 3. Pinkus et al. Human Pathol 16: 929, 1985.