Technical Data
E3414-22
Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)
Description:
Applications:
Suitable for use in Immunoblot and Immunohistochemistry (frozen and paraffin-embedded tissue; Proteolytic treatment not required).

Recommended Dilution:
Immunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F80Ascites
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Isolated from primary breast carcinoma cells.
Purity:
Ascites
Form
Supplied as a liquid in PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Reactive with Epithelial Sialomucin, a mucous glycoprotein located mainly in the glycocalyx of most glandular epithelial cells and in carcinoma cells. Directed against a 400kD glycoprotein, corresponding to MAM-6. Occasionally, a very weak crossreactivity with sarcomas and brain tumors is observed. It does not react with normal colon or rectal epithelium and colorectal adenomas, whereas colorectal carcinomas stain positive. Does not crossreact with leukemias. Reactive with human. Recommended for Mamma carcinoma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hageman et al. In: Application of monoclonal antibodies in tumour pathology. D. Ruiter, G J Fleuren and S O Warnaar, Martinus Nijhoff pp. 167-190 (1987).