		Technical Data
E3440-05	Epstein Barr Virus (EBV)

Description:
The viral capsid antigen of EBV is abundantly expressed in cells undergoing productive infection, and it has polypeptide and glycoprotein components ranging in size from 26-200kD, with a 143kD polypeptide (BNRF1 reading frame) being the major component. The membrane antigen complex is found on the virion envelope and consists of at least three major EBV induced glycoproteins: gp350/220, gp125, and gp85. gp125, encoded by BALF4, is found in abundance in the cell nucleus and in the endoplasmic reticulum but it is not a major structural protein of the virion.

Applications:
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Antigen retrieval is required
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	1.B.263	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~0.1mg/ml

Immunogen:

Recombinant EB nuclear antigen.

Purity:

Purified by Protein A affinity chromatography

Form

Supplied as a liquid PBS, pH 7.2, 0.1% sodium azide.

Specificity:

Recognizes the EB nuclear antigen (EBNA-1) at ~70kD. Reacts with the carboxy terminus (~last 100aa) of the molecule.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.