		Technical Data
E3440-10K	Epstein Barr Virus, Early Antigen Diffuse (EBV EAD, Human Herpesvirus 4, HHV4)

Description:
Epstein Barr virus early antigens are non-structural pro- teins that do not require viral DNA replication for synthesis. This antibody detects early antigen diffuse during the lytic phase of virus replication.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
B95-8 cell line

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	G3-E31	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice		Mouse

Concentration:

Not determined

Immunogen:

B95-8 cell lysates treated with phorbol myristic acid (PMA).

Purity:

Supernatant

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

Specificity:

Recognizes EAD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Thomas J A, Felix D H, Wray D, et al.. Epstein-Barr virus gene expression and epithelial cell differentiation in oral hairy leukoplakia. American Journal of Pathology. 139(6):1369-1380 (1991). 2.Miller G. Epstein-Barr Virus-Biology, pathogenesis, and medical aspects. Virology, Second Edition. 1921-1958 (1990).